Detection of rfbj(B) gene of Salmonella serogroup-B isolated from patients with salmonellosis
Kerbala Journal of Medicine,
2012, Volume 5, Issue 12, Pages 1413-1419
Abstractsummary: A total of 480 fecal samples were collected from patients (less than 18 years old), of both sex suffering from diarrhea who admitted to Al-Diwaniya Teaching Hospital and the Teaching Hospital of Maternity and Pediatrics in Al- Qadisiya governorate. Salmonella spp. were isolated and identified using bacterial culturing on selective media, in addition to, biochemical and serotyping by monovalent antisera. Polymerase Chain Reaction (PCR) was used to detect rfbj(B)gene encoding for biosynthesis of LPS of group B Salmonella. The results revealed that the rate of Salmonella isolates in fecal samples of patients were 7.9% using cultural and biochemical methods .The result of Salmonella isolates by serotyping using monovalent antisera revealed that 30 out of 34 isolates (88 %) belong to S. Typhimurium serotype , while the remain belong to S. Enteritidis ( 2 isolates ) and S.Meunchen (2isolates). when the PCR technique was used to detect the presence of rfbj(B) gene, 34 Salmonella isolates belong to Salmonella serogroup (B) appeared to contain this gene since DNA amplification showed one distinct band (882 bp) when electrophorised on agarose gel. The results of this study revealed that the PCR technique had a high specifity (100%) in detection of Salmonella serogroup B in comparison to cultural, biochemical and serological tests.
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