Isolation, Purification, And Selection Criteria Of Probiotic Bacteeria “Lactobacillus Jensenii
Kerbala Journal of Medicine,
Volume 5, Issue 11, Pages 1211-1218
Abstractlactobacilli isolated from stool samples of healthy infants aged 2-28 days were identified as Lactobacillus jensenii based on its morphological description on MRS agar and blood agar, Gram stain, catalase test, and results of API 20 A system . The Lactobacilli were assessed for characteristics considered important for in vitro resistance ability to low pH ( 3,4,5, and 6 ), and resistance ability to different bile salt concentrations 0.5%,1%,1.5%,2%,2.5%, and 3% ). The 'other characteristics studiedwere culture and sensitivity of probiotic bacteria to different types of antimicrobial and antibiotics. The probiotic bacteria revealed inhibitory activities against several pathogenic bacteria (Staphylococus aureus, Streptococcus agalactia, Clostridium perifrenges, Pseudomonas aeroginosa, Escherichia coli, Klebsiella pneumonia,'Salmonella typhi, Vibrio cholerae, and Shigella flexneri ):. Lactobacillus jensenii alsoexhibited its ability for attachment to epithelial cells of human intestine (in vitro).
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