Immune response to heat shock protein 60 and its relations t enteric reactive arthritis
Kerbala Journal of Medicine,
Volume 2, Issue 5, Pages 732-736
Abstractbackground: according to the molecular mimicry hypothesis, heat shock
protein 60 (hsp60) is among the most conserved proteins that have been
implicated as triggering agents in the pathogenesis of enteric reactive arthritis
Objective: In the present work, we aimed to determine the prevalence of anti-hsp60
antibodies in sera of patient with enteric reactive arthritis.
Methods: Forty-five patients with Reactive arthritis were enrolled in this study. They
were 22(48.89%) males and 23(51.11%) females, the age rang was 20 - 40 year with
mean (33.6 ± 10.6). All patients were outpatient visitor or hospitalized in the medical
City hospital in Baghdad. They were diagnosed clinically by consultant rheumatologist
with the aid of some laboratory tests such as RF, CRP, E.S.R.
The patients should be sero-negative (RF-negative) and fulfill Amor and
European Criteria to be included in this study. Patients were classified according to
disease activity into three groups: sever moderat and mild by using (DAREA score).
Thirty age and sex matched apparently healthy individual, were considered in this
study as a control group. ELISA was used to detect immune response against hsp60 in
the sera of each patients and controls. Wells of the micro titter plates were coated with
hsp60 in coating buffer and anti hsp60 antibodies were assayed.
Results: the mean age of patients was (33.6± 10.6) years and they were 23 females
and 22 males with females to male ratio 1.05:1, the majority of patients 19(42.22%)
present with high disease activity (sever) and 15(33.33%) patients were moderate and
the remainder 11 (24.44%) were mild disease group.
The result of anti-hsp60 Abs detection showed that there was highly significant
difference between ReA patients and control groups. Also there was significant
difference between sever, moderate and mild among ReA patients.
Conclusion: we concluded that bacterial hsp60 seems to be a major target of T-cell
response in enteric ReA. and cross reactivity against autologous hsp60 has been
documented as a triggering of enteric ReA.
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