Zinc, Copper, and Superoxide Dismutase in Spermatozoa of Patients with Asethenospermia
Kerbala Journal of Medicine,
2009, Volume 2, Issue 4, Pages 420-428
Abstractobjective:To determine the level of Superoxide dismutase (SOD), Zinc and Copper in
the seminal and spermatozoal of patients with asthenospermia.
Setting: All semen samples were collected in the infertility unit of the laboratory (in
Amel clinic for detection and treatment of infertility/Iraq. Hilla city) after a 3-5 day period of
sexual abstinence, then incubated at 37°C and analyzed within 1 h.
Methods:The study was conducted on 30 patients and compared to 20 controls. Statistical
analysis between group 1 (controls) and group 2 (patients) was performed by the student's t -
test. Zinc and copper content was assayed by atomically spectrophotography,SOD enzyme was
measured by biochemical assay. This work was undertaken to assess oxidative stress and
Results: It was observed that there was a significant increase in seminal and spermatozoal
superoxide dismutase activity and copper content. While Zinc were significantly decreased.
Conclusions :The results of this study suggest higher oxygen-free radical
production, evidenced by increased superoxide dismutase activities, support to the
oxidative stress in asthenospermia. The increased activities of antioxidant enzyme may be a
compensatory regulation in response to increased oxidative stress.
Keywords:Zinc,Copper,Superoxide dismutase(SOD),Human sperm
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