Morphological Study of the mice’s Islets of Langerhans and β-cells mass assessment during Pregnancy and lactation conditions
Kerbala Journal of Medicine,
2016, Volume 9, Issue 2, Pages 2523-2533
ackground: Islets of langerhans adapts to changing insulin demands in the body. One of the most amazing reversible islets of langerhansadaptations occurs during pregnancy and postpartum conditions. During pregnancy,β-cells mass expand by increasing their number and size . and they are rapidly reversed at end of pregnancy by β- cell apoptosis and retained to normal level .
Objectives: This study attempt to demonstrate the changes of the islets of langerhans that happen during pregnancy and lactation including changes in the general morphological and histological features, changes in the certain islets’ parameters e.g. number ,diameter and mean areas, and changes in the β – cells mass.
Material and Methods: We were used thirty female mice which divided into (3) groups ten for each group. Group(A): served as a control. Group(B) was pregnant group (at day 15 of gestation). Group(C) was lactating group (at day 4 of Postpartum). Tissues were processed for both paraffin block and semithin plastic sections . Tissue sections were stained with H&E stain, Gomoritri chrome stain and NDS. Two digital image analysing soft wares were used in this study: Image J program and Image Scope program
Results: It was demonstrated that the formation of new islets, enlargement of islets by union of adjacent small islets and by increased their cellularity were common features of pregnant group. also it was showed that in postpartum group, apoptosis of beta -cells started to restore the beta- cell number to normal level. The morphometric analysis of the islets parameters in this study showed a highly significant differences among the studied groups with P value ≤ 0.001. The mean number , mean diameter and mean area of islets were significantly higher in pregnant group, intermediate in lactating group and lowest in control group. In this study, the result also showed that the β-cell mass rose near the doubled during pregnancy as compared with control group, then decreased by 25% at 4th day of postpartum. This study conclude that islets of langerhans subjected to natural compensatory changes during pregnancy then they retain to normal state when pregnancy ended
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