Study of Immune Adjuvant Activity of Propolis against MRSA in Laboratory Animals
Kerbala Journal of Medicine,
Volume 9, Issue 2, Pages 2513-2522
Abstractbackground: Propolis is a natural resinous product that honeybees collect from several plants and mix it with bees wax and salivary enzymes that is also called bee glue. Propolis has many properties that make it is chief in the medicine including antibacterial, antiviral, antifungal, antitumor and immunodulatory properties.
Aim: The aim of this study was to evaluate immune adjuvant activity of propolis against Methicillin Resistant Staphylococcus aureus MRSA in vivo.
Materials and Methods: Twenty male white New Zealand rabbits included in this study, that divided into tow groups (10 rabbits at each one), group I received orally one milliliter of Ethanolic Exract Propolis (EEP) at 20% concentration per day for lasting 20 days, while group II have no received any thing as control group. After 10 days from last dose of adminstration of EEP, all animals injected in intraperitonial (IP) with six doses of 108 cfu/ ml of killed somatic antigen of Staphylococcus aureus. Blood samples were taken by heart puncture from all animals to detect the immunological parameters by ELISA technique.
Results: Results revealed to significantly increased the mean value of total leukocytes counts (TLCs) in group I (5705 cells/mm3) as comparing with group II (4600 cells/mm 3), at level p ˂ 0.05, as well as the results pointed to increased significantly the percentage of neutrophil cells in group I (42%) as compared with group II (34%), in contrast to lymphocytes results that show significantly decrease in group I ( 54% ) as compared with group II ( 61%), in addition, monocytes, eosinophil and basophil were have no differences among the testing groups. Phagocytic activity result shows increased significantly in group I (55.47%) as compared with group II (43.94%). The results of complement component C3 revealed increased significantly in group I (69.76 pg/mL) as compared with group II (18.61 pg/mL), while the results of C4 concentration pointed to increase significantly in group I (4.20 pg/ml) as compared with group II (2.38 pg. /ml). IFN-γ concentration results revealed increased significantly in group I (22.18 pg/mL) as compared with group II (18.05 pg/mL. IL-2 results referred to increased significantly in group I (32.42 pg/mL) as compared with group II (24.27 pg/mL). On the other hand, the level of perforin was increased significantly in group I (0.23 ng/ mL) as compared with group II (0.12 ng/ mL).
Conclusion: It was concluded that propolis improve immune responses as adjuvant against MRSA antigen via increase the levels of cellular immunity elements.
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