Assessment of Genetic Variations Associated With Susceptibility to Psoriasis Among Iraqi Population
Kerbala Journal of Medicine,
2015, Volume 8, Issue 1, Pages 2049-2055
Abstractbackground: Psoriasis is an immune –mediated, chronic hyperproliferative disease ofthe skin. Genetic association studies have identified multiple psoriasis risk loci;however, the frequency of variations at these loci differs quite strikingly among the populations, suggesting heterogeneity in the genetic susceptibility to psoriasis.
Aim of the study: to assess the possible association of selected genetic variants with psoriasis among Iraqis.
Methodology: In this case-control study, 50 psoriasis patients and 40 control subjects were enrolled. Peripheral blood was obtained from all patients and control subject and used for extraction of genomic DNA. Amplification Refractory Mutation System (ARMS- PCR) was used to detect 3 SNPs in IL-20RA(Rs1184860, Rs1167846 and Rs1167849), whereas convention PCR was used to assess deletions in late cornified envelope 3B and late certified envelope 3C (LCE3B and LCE3C) genes.
Results and Discussion: Nine haplotypes were identified by SNPs atIL-20RA gene; the haplotype TTA (Rs1184860, Rs1167846 and Rs1167849, respectively) was associated with psoriasis 19(38%), whereas the CTA haplotype had a protective effect 12(30%). The frequencies of LCE3B deletion in both patients and control groups were 14(28%) and 20(50%) respectively, while the frequencies of LCE3C deletion were 16(32%) and 20(50%) among patients and control respectively. Statistical analysis revealed a significant association between LCE3B deletion and susceptibility to the psoriasis (P < 0.05). The frequencies of homozygous deletion (LCE3B_LCE3C del) in both patients and control groups were 13(26%) and 9(22.5%) respectively. The statistical analysis had revealed a non significant (P > 0.05) association between LCE3C deletion and homozygous deletion (LCE3B_LCE3C del) with susceptibility to psoriasis.
Conclusion:The haplotype TTA of IL-20RA gene has a role in the susceptibility to the psoriasis among Iraqi population.In addition, no association was found between LCE3B_LCE3C deletion and psoriasis in Iraqi population.
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