Keywords : antibody titration


Prevalence of Anti-Rh (D) Antibody in Karbala

Mohammed Shnain Ali

Kerbala Journal of Medicine, Volume 6, Issue 2, Pages 1644-1647

background: Neither Rh(D) positive individuals nor Rh (D) negative individuals
normally have anti-Rh(D) antibody in their blood; however, Rh (D) negative
individuals have the ability to form such antibody when they are transfused with
Rh(D) positive blood.
Objective: To determine the prevalence of anti-Rh (D) antibody in Karbala.
Patients and methods: From January 2012 to March 2013, 226 patients (127 males and 99
females with age range of 20-49 years) were studied after their consent. For each patient, 4
ml of venous blood sample was obtained and investigated as follows:
Two ml of blood were transferred to EDTA tube for ABO and Rh(D) grouping using the
commercially available kit (monoclonal anti-A, anti-B and anti-D) by doing the ordinary tile
method (equal volumes of blood and reagent were mixed on tile looking visually for the
agglutination for positive reaction and positive result. Absence of agglutination means
negative reaction and negative result). The other 2 ml of blood were transferred to plain tube,
centrifuged and serum is investigated for anti-Rh(D) antibody in Rh(D) negative individuals
by double dilutions of each serum and antibody titration method.
Results: Out of 226 individuals, 186 (82.3%) were Rh(D) positive, 40 (17.7%) were Rh(D)
negative, and 2 were found to be positive for anti-Rh(D) antibody. The prevalence of anti-
Rh(D) antibody is 0.88%.
Conclusion: The study showed that the prevalence of anti-Rh(D) antibody in Karbala is
0.88%.